3 edition of Pluripotent stem cells found in the catalog.
Pluripotent stem cells
Includes bibliographical references and index.
|Statement||editors, Derek W. Rosales and Quentin N. Mullen.|
|Contributions||Rosales, Derek W., Mullen, Quentin N.|
|LC Classifications||QH588.S83 P58 2009|
|The Physical Object|
|LC Control Number||2009048917|
The book covers the derivation of human stem cell lines, the obtaining of cells from human stem cell banks, the culturing and characterisation of the cells, and the differentiation of the cells in vitro and in vivo. Lastly, almost all of these protocols can also be used for analyzing and manipulating induced pluripotency iPS stem cells. The embryonic stem cell-like clones were called "iPS" cells (Induced Pluripotent Stem Cells) and further investigations are needed to determine if adult somatic cells can be converted to iPS cells using Oct4 and Sox2 and if such cells can be efficiently induced to differentiate into the desired types of cells for medical uses.
Pluripotent stem cells have the ability to undergo self-renewal and to give rise to all cells of the tissues of the body. However, this definition has been recently complicated by the existence of distinct cellular states that display these features. Here, we provide a detailed overview of the family of pluripotent cell lines derived from early mouse and human embryos and compare Cited by: These stem cells are called totipotent or pluripotent stem cells. There’s a slight difference between the two words: totipotent means that the stem cell can give rise to any and all human tissue cells – and it can even give rise to an entire functional human.
hematopoietic stem and progenitor cells for research use only. not intended for human or animal diagnostic or therapeutic uses. 3 stemcell technologies inc.s ualit management sstem is certified to iso medical device standards. ISBN: OCLC Number: Description: x, pages: illustrations (some color) ; 26 cm. Contents: 1. Sources and derivation of human embryonic stem cells / Michal Amit New chemical approach to the efficient generation of mouse embryonic stem cells / Hossein Baharvand and Seyedeh-Nafiseh Hassani Review of the methods for .
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28 rows This book is a collection of studies/reviews, beginning with an introduction to the pluripotent stem cells and covering various aspects like derivation, differentiation, ethics, etc., and hence would provide insight into the recent standing on the pluripotent stem cells by: 1.
Stem cell research is a rapidly expanding field with the potential to develop therapeutic agents to treat diseases as well as study disease development from early stages.
The culture of human pluripotent stem cells shares many of the same protocols as standard mammalian cell culture. Organized in two volumes entitled Pluripotent Stem Cells and Cell Biology and Adult and Fetal Stem Cells, this work contains contributions from the world’s experts in stem cell research to provide a description of the tools, methods, and experimental protocols needed to study and characterize stem cells and progenitor populations as well as a.
Pluripotent stem cells represent an attractive alternative to tissue specific stem cells as they have unlimited proliferation capacity and the ability to differentiate into all somatic cell lineages.
Human pluripotent stem cells were first described in when James Thomson and colleagues isolated embryonic stem (ES) cells from the inner cell mass of blastocyst stage human embryos. This volume aims to be a collection of essential protocols in iPS cell field.
Induced Pluripotent Stem (iPS) Cells: Methods and Protocols guides readers through multiple facets of stem cell biology, lineage commitment and differentiation. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials Format: Hardcover.
Embryoid bodies (EB) are the three-dimensional aggregates formed in suspension by pluripotent stem cells (PSC), including embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC). EB differentiation is a common platform to generate specific cell lineages from PSCs.
Directed Differentiation and Disease Modeling. Quality Control. Product Types. Cell Culture Media and Supplements. 24 rows Pluripotent stem cells have distinct characteristics: self-renewal and the potential to Author: Minoru Tomizawa.
Pluripotent cells can give rise to all of the cell types that make up the body; embryonic stem cells are considered pluripotent. Multipotent cells can develop into more than one cell type, but are more limited than pluripotent cells; adult stem cells.
Pluripotent Stem Cells. Few areas of biology currently garner more attention than the study of human pluripotent stem cells (hPSCs).
This interest has arisen because of their potential to form the basis of cellular therapies for diseases affecting organ systems with limited regenerative capacity, to provide enhanced systems for drug screening and toxicity testing as well as to.
Pluripotent Stem Cells: Methods and Protocols highlights the best methods and systems for the entire work flow. Divided into four convenient sections, topics include a focus on producing iPSC from diverse somatic sources, media systems for expanding ESC and iPSC with detailed protocols for directed differentiation into specific lineages.
This volume aims to be a collection of essential protocols in iPS cell field. Induced Pluripotent Stem (iPS) Cells: Methods and Protocols guides readers through multiple facets of stem cell biology, lineage commitment and differentiation. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials.
“This book is a collection of methods and protocols for the successful generation and characterization of pluripotent stem cells. It will be helpful to researchers, lab technicians, and students interested in creating pluripotent cells and their commitment to specific cell lineages.
Pluripotent stem cells (PSC) can divide indefinitely, self-renew, and can differentiate to functionally reconstitute almost any cell in the normal developmental pathway, given the right conditions. This comprehensive book, which was developed from a training course, covers all of the PSCs (embryonic, embryonic germ, and embryonic carcinoma) and.
Hematopoietic Differentiation of Human Pluripotent Stem Cells (SpringerBriefs in Stem Cells Book 6) - Kindle edition by Tao Cheng. Download it once and read it on your Kindle device, PC, phones or tablets. Use features like bookmarks, note taking and highlighting while reading Hematopoietic Differentiation of Human Pluripotent Stem Cells (SpringerBriefs in Stem Cells Book.
Stem Cells. Mar;35(3) doi: /stem Epub Feb 5. Concise Review: Induced Pluripotent Stem Cell Research in the Era of Precision by: Second, pluripotent stem cells can potentially be customized to provide a perfect genetic match for any patient.
This means that patients could receive transplants of tissue and cells without tissue matching and tissue rejection problems, and without the need to take powerful immune-suppressing drugs for the rest of their lives. Genome editing is used to make targeted modifications to the genome of eukaryotic cells.
There are many potential applications of genome editing in human pluripotent stem cells (hPSCs) including the generation of knockout and reporter cell lines. This protocol describes a system for efficient genome editing in hPSCs using engineered transcription Cited by: Induced pluripotent stem cells (iPSCs) are adult cells that have been genetically reprogrammed to an embryonic stem cell–like state by being forced to express genes and factors important for maintaining the defining properties of embryonic stem cells.
Although these cells meet the defining criteria for pluripotent stem cells, it is not known. Pluripotent stem cells are master cells.
They’re able to make cells from all three basic body layers, so they can potentially produce any cell or tissue the body needs to repair itself. This “master” property is called pluripotency. Like all stem cells, pluripotent stem cells are also able to self-renew, meaning they can perpetually. This book is a compilation of studies, starting with an introduction to the pluripotent stem cells and encompassing insightful information regarding the latest standing on the pluripotent stem cells biology and elucidation of the potential therapeutic implications and ethical concerns of these in vitro developed cells in different diseases.
Human pluripotent stem cell: One of the "cells that are self-replicating, are derived from human embryos or human fetal tissue, and are known to develop into cells and tissues of the three primary germ layers.
Although human pluripotent stem cells may be derived from embryos or fetal tissue, such stem cells are not themselves embryos." (From the National Institutes of .Contents Introduction 4 1 Reprogramming of human fibroblasts 7 2 Selection of iPSC clones for feeder-dependent culture 21 3 Preparation of feeder layer 25 4 Feeder-dependent culture of human induced pluripotent stem cells (hiPSCs) 29 5 Transition of mature iPSCs to feeder-free culture system 39 6 Culture, expansion, and cryopreservation of pluripotent stem cells in .